TGF-B ligands bind receptors consisting of type 1 and type 2 serine/threonine kinases, which serve to propagate the signal intracellularly. Ligand binding stabilizes a receptor complex consisting of two type 1 receptors, and two type 2 receptors. Type 2 receptors then can phosphorylate type 1 receptors at locations on the GS domain, located N-terminally to the type 1 kinase domain. This phosphorylation event activates the type 1 receptors, making them capable of further propagatin… WebSmads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. The Smad family is …
Smad3 and phospho-Smad3 are potential markers of invasive ...
WebThe Phospho-Smad5 (Ser463,Ser465) Antibody has been validated for the following applications: Western Blot. Western Blot analysis of Phospho-Smad5 (Ser463,Ser465) performed on various cell lines using a Phospho-Smad5 (Ser463,Ser465) monoclonal antibody (Product # MA5-35867) at a dilution of 1:1,000. An HRP Goat Anti-Rabbit IgG … WebApr 24, 2008 · The anti-phospho-Smad2 antibody specifically recognized the phosphorylated form of Smad2, whereas the anti-phospho-Smad1/5/8 antibody also reacted to the unphosphorylated form. These antibodies did not react with the other Smads. TGF-β Transforming growth factor-β BSA Bovine serum albumin. Keywords Smad … small part synonym
National Center for Biotechnology Information
WebAnti-phospho Smad1/Smad5/Smad8 (Ser463/465) Antibody, Cat. No. AB3848-I, is a highly specific rabbit polyclonal antibody that targets Smad1//5/8 phosphorylation and has been … WebPhospho-SMAD1/SMAD5 (Ser463, Ser465) Antibody (700047) in ICC/IF Immunofluorescence analysis of SMAD1/5 (pS463/465) was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 … WebIn response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. small parts warehouse storage