Phospho-akt1/2/3 ser473 cellular kit

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August undefined, 2024

WebPhospho-Akt (Ser473) Kit For use with human, mouse, rat, and non-human primate cell lysates and tissue lysates. This package insert must be read in its entirety before using … WebThe protocol is simple and straightforward, with just two incubation steps: - Cell stimulation by the target compounds - cAMP detection using HTRF reagents Product specifications Designed with signal performance in mind, the kit offers a tailored standard curve ideally suited to the study of Gi coupled GPCRs. Introduction to product validation

AlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kits, PerkinElmer

WebAKT is a serine/threonine protein kinase that plays a key role in multiple cellular processes including metabolism, proliferation, apoptosis/survival, and migration. There are three homologous isoforms of AKT: AKT1, AKT2, and AKT3. AKT is activated by binding of its pleckstrin homology (PH) domain to membrane phospholipids and by phosphorylation. WebSep 15, 2024 · The protein concentration of the supernatants was measured by BCS TM protein assay kit according to manufacturer’s ... The knockout of STK24 expression did … on the flat

Phospho (Ser473)/Total Akt Whole Cell Lysate Kit

WebPhospho-Akt (Ser473) Rabbit mAb has been coated on the microwells. After incubation with cell lysates, phospho-Akt (Ser473) protein is captured by the coated antibody. Following extensive washing, Akt1 Mouse Antibody is added to detect the captured phospho-Akt1 (Ser473) protein. Webphospho-AKT 1/2/3 (Ser473) Detection Antibody Reagent Positive Control Cell Lysate (lyophilized) Detection Reagent (TMB Substrate) Stop Solution Plate seal Description … WebSep 12, 2015 · Inhibition CellularAkt semisyntheticviridins epidermalgrowth factor–dependent activation HT-29colon cancer cells Westernhybrid- ization using anti-phospho-Ser 473 -Akt antibody (Fig. 2A). Quantitation phospho-Ser473 totalAkt (Fig. 2B) gave IC 50 values (FSE) 2.2nmol/L, PX-867 3.5nmol/L, on the flash drive

AlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kits, PerkinElmer

AlphaScreen® SureFire® Akt 1/2/3 (p-Ser473) Assay …

Web寻找 Abcam 抗体及试剂 - Abcam中文官网 WebPhospho-AKT1/2/3 (Ser473) control lysate HTRF®. Phospho-AKT1/2/3 (Ser473) control lysate. This lysate is a component of the phospho-AKT1/2/3 (Ser473) kit. It may be used … on the flash who is savitarWebThe Total SHP1 cellular assay monitors the expression level of SHP1, and can be used as a normalization assay with our phospho-SHP1 Y564 kit. Because these phospho and total SHP1 assays are compatible, the two kits can be used in parallel on the same lysates. Many cancer cells overexpress checkpoint inhibitor ligands such as PD-L1. on the fleeing from the cops

"LANCE Ultra phospho-AKT1/2/3 (Ser473) kits are designed for the detection of AKT1, AKT2, and AKT3 (phosphorylated at residue S473) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells. " - Phospho-akt1/2/3 ser473 cellular kit

Phospho-akt1/2/3 ser473 cellular kit

Phospho-Akt (Ser473) Antibody Cell Signaling Technology

WebPhospho-AKT1 (Ser473) Antibody (700392) in ICC/IF Immunofluorescent analysis of AKT (pS473) was done on 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. WebThe Phospho-AKT (Ser473) kit is designed to robustly and efficiently monitor AKT phosphorylation on Ser473 across all isoforms, as a readout of Pi3K pathway activation. …

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WebUp-regulation of O-GlcNAcylation increases Akt1 phosphorylation at Ser473. The 8305C cells were treated with 5 μM Thia-G or were transfected with 500 ng OGT plasmid, and then cell lysates were resolved by SDS-PAGE, and phospho-Akt1 (Ser473) or Akt1 were determined with specific antibodies (Figure 4A and B). An increase in Ser473 ... Web#IR172-668 / IR172-668,Phospho-AKT (Ser473) antibody. Products ... and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. ... Subsequent phosphorylation of both threonine residue 308 and serine ...

WebPhospho-AKT1/2/3 (Ser473) cellular kit HTRF® Kit Diabetes and metabolic disorders A portfolio to tackle in-depth diabetes and metabolic disorders studies Explore Phospho-AKT1/2/3 (Ser473) control lysate HTRF® Control lysate Phospho-AKT1/2/3 (Thr308) cellular kit HTRF® Kit Phospho-AKT1/2/3 (Thr308) control lysate HTRF® Control lysate Guides WebFeb 3, 2024 · Protein kinase B (AKT1) is hyper-activated in diverse human tumors. AKT1 is activated by phosphorylation at two key regulatory sites, Thr308 and Ser473. Active AKT1 phosphorylates many, perhaps hundreds, of downstream cellular targets in the cytosol and nucleus. AKT1 is well-known for phosphorylatin …

Web(Ser473) Chemiluminescent Sandwich ELISA Kit detects endogenous levels of phospho-Akt1 (Ser473) in human, rat and mouse cells. Please visit www.cellsignal.com for a complete listing of recommended companion products. #7134C PathScan® Phospho-Akt1 (Ser473) Chemiluminescent Sandwich ELISA Kit Species Cross-Reactivity: H, M, R … WebThe Phospho-AKT3 (Ser473) kit is designed for the robust quantification of AKT3 modulation, phosphorylated on Ser473. Also known as protein kinase B (PKB), AKT is an …

WebAKT, also known as protein kinase B, is an important regulator of numerous cellular processes including cell survival, glucose metabolism, transcription, and apoptosis. AKT …

WebThis assay kit contains antibodies which recognize the phospho-Ser473 epitope, and a distal epitope, common to Akt 1, Akt 2 and Akt 3. The proteins detected by this kit correspond to … on the flats on the road to cribb island ion shampoo sally beauty supplyWebThe Phospho-AKT3 (Ser473) kit is designed for the robust quantification of AKT3 modulation, phosphorylated on Ser473. Also known as protein kinase B (PKB), AKT is an oncogene playing a key role in controlling apoptosis, cell proliferation, transcription, cell migration, and glucose metabolism. ions have a different number of whatWebThe phospho-AKT1 (Ser473) kit is designed for robust quantification of AKT1 modulation, phosphorylated on Ser473. Also known as protein kinase B (PKB), Akt is an oncogene … ions hair productsWebAlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kits, PerkinElmer Supplier: Perkin Elmer Harmonized Code US-3822005090 The AlphaLISA® SureFire® Ultra™ p-AKT1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Order Now … on the fleeing from the police crosswordWebNov 25, 2015 · The ER also plays a critical role toward maintenance of cellular calcium homeostasis. ... and the following oligonucleotide primers were used for RT-PCR: Akt1 forward, 5′-CTGTCTCGAGAGCGTGTGTT-3′; Akt1 reverse, 5′-AACAGCTTCTCGTGGTCCTG-3′; Akt2 ... [rabbit polyclonal anti-phospho-Akt (Ser473) antibody (Cell Signaling Technology, … on the fleeingWebPhospho-ERK assay kits are designed for the robust quantification of ERK modulation, phosphorylated on Thr202/Tyr204, for GPCR and RTK targeted screening as a MAPK … on the fleeing daily themed crossword