Phic31 integrase system
Web25. nov 2024 · Here, we adapted the phiC31 integrase system for C. elegans. We generated an integrated phiC31 integrase expressing strain flanked by attP sites that also serves as a landing pad for... WebThe PhiC31 Integrase System Popularly used for gene therapy development and related applications, one of the major advantages of SBI’s PhiC31 system is the tight control over copy number—the majority of clones that undergo integration events have single insertions (Supporting Data, Figure 1), reducing the risks of insertional mutagenesis effects.
Phic31 integrase system
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WebWe previously established that the phage phiC31 integrase, a site-specific recombinase, mediates efficient integration in the human cell environment at attB and attP phage attachment sites on extrachromosomal vectors. Web25. júl 2024 · The PhiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage PhiC31. The PhiC31 integrase mediates precise, unidirectional recombination between two att sites named attB and attP. This integrase has been shown to function efficiently in many different cell types as well as in mice [ 12, 13 ].
WebPhiC31 integrase is a serine recombinase containing an N-terminal domain (NTD) that provides catalytic activity and a large C-terminal domain that controls which pair of DNA substrates is able to ... Web1. jan 2007 · The phiC31 integrase can integrate introduced plasmid DNA into preferred locations in unmodified mammalian genomes, resulting in robust, long-term expression of …
Web1. feb 2005 · PhiC31 integrase system is considered as a specific tool for gene therapy (3, 4) and transgenic research (2,5). The efficiency of phiC31-integrase has been indicated to be comparable with that of ... WebIt is known that phiC31 integrase has the capacity to mediate recombination between the target sequences attP and attB. To test the availability of site-specific integration in the …
WebThe objective of the present experiment was to evaluate PhiC31-based site-specific transgenesis system for production of transgenic bovine embryos by somatic cell nuclear transfer and intracytoplasmic sperm injection. Materials and Methods: In this experimental study, the application of phiC31 integrase system was evaluated for
WebTo overcome these problems, it is necessary to develop a site-specific integration system. It is known that phiC31 integrase has the capacity to mediate recombination between the target sequences attP and attB. To test the availability of site-specific integration in the silkworm, we first examined the efficiency of recombination between the ... how to do antilog on desmosWebThe phiC31 integrase system represents a novel technology that opens up new possibilities for gene therapy. The phiC31 integrase can integrate introduced plasmid DNA into … how to do antilogWeb17. sep 2008 · The phiC31 integrase system is expected to be functional in other monocotyledonous species and in commercial wheat varieties. We succeeded in transforming phiC31 integrase vectors into rice ( Oryza sativa ), pearl millet ( Pennisetum glaucum ) and varieties of commercial winter wheat provided by the Nordsaat Saatzucht … how to do anthropometric measurementsWeb18. máj 2024 · Use of phage PhiC31 integrase as a tool for zebrafish genome manipulation. In: Detrich, H.W., Westerfield, M., and Zon, L.I., eds. Methods in Cell Biology. Zebrafish: Genetics, Genomics and Informatics, 3rd Ed. New … the natterjack pubhow to do antilog on casio calculatorWebThe phiC31 integrase system efficiently integrates plasmid DNA carrying a transgene and an attB site into a limited number of endogenous pseudo attP sites in mammalian genomes, leading to robust ... the natty hourWeb14. sep 2012 · The Streptomyces phage phiC31 integrase was tested for its feasibility in excising transgenes from the barley genome through site-specific recombination. We produced transgenic barley plants expressing an active phiC31 integrase and crossed them with transgenic barley plants carrying a target locus for recombination. The target … how to do antilog on ti-30xa