WebphiC31 integrase is a site-specific recombinase from a bacteriophage that has become a useful tool in mammalian cells. The enzyme normally performs precise, unidirectional … WebHere we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane …

Using a phiC31 “Disintegrase” to make new attP sites in the …

Webthrough recombination at sites with sequence similarity to the native attP site (termed pseudo-attP sites). phiC31 integrase can integrate a plasmid of any size, as a single copy, … Web17. mar 2006 · The phiC31 integrase catalyzes precise, unidirectional recombination between its 30-40-bp attP and attB recognition sites. In mammalian cells, the enzyme … nursing care plan bipolar disorder

Generation of Drosophila attP containing cell lines using CRISPR …

Web8. sep 2013 · Phage PhiC31 integrase integrates attB-containing plasmid into pseudo attP site in eukaryotic genomes in a unidirectional site-specific manner and maintains robust … Web1. aug 2014 · Introduction. I n nature, the phage φC31 integrase recombines an attP site in the phage genome with a chromosomal attB site of its Streptomyces host (Andreas et al., 2002).Mammalian genomes were found to contain “pseudo” attP sites, which have partial sequence identity to the phage attP site and can also mediate efficient integrase activity … WebThe phiC31 integrase catalyzes unidirectional recombination between two attachment sites, attP and attB, leading to the formation of attL and attR sites . A circular construct harboring an attB site can be efficiently and specifically integrated into … nursing care plan chest pain