Cytokinin dehydrogenase activity

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August undefined, 2024

WebThe Cytokinin Oxidase/Dehydrogenase CKX1 Is a Membrane-Bound Protein Requiring Homooligomerization in the Endoplasmic Reticulum for Its Cellular Activity Degradation … WebModifying the cytokinin content in plants is a means of improving plant productivity. Here, we report the development and biological activity of compound TD-K (1-(furan-2-ylmethyl)-3-(1,2,3-thiadiazol-5-yl)urea)which is related to thidiazuron. TD-K—which exhibited extremely high antisenescence activity in the wheat leaf bioassay—and INCYDE (2 …

Cytokinin oxidase/dehydrogenase activity as a tool in …

WebApr 11, 2024 · 1.Introduction. Zinc (Zn), as one of the essential trace elements, regulates the activity and conformational stability of various proteins and enzymes by acting as a catalyst and regulatory or structural cofactor (Coleman, 1998).In plants, approximately 2,400 proteins require activation by Zn 2+ (Sinclair and Krämer, 2012), such as Zn-finger DNA … WebJul 1, 2002 · Two novelSpectrophotometric methods for determining the activity of cytokinin oxidase/cytokinin dehydrogenase (EC 1.5.99.12) were developed and optimized and can be used for kinetic studies with purified enzyme and is entirely substrate independent. Spectrophotometric methods for determining the activity of cytokinin … react fast refresh not working

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WebWendy A. Stirk mainly investigates Botany, Cytokinin, Axenic, Chlorophyceae and Zeatin. Wendy A. Stirk has included themes like Micropropagation and Tissue culture in her Botany study. ... antioxidant and antibacterial activity of Hypoxis hemerocallidea Fisch. & C.A. Mey (28 citations) ... Her work deals with themes such as Malate dehydrogenase ... Webcytokinin oxidase activity in the presence of copper-imidazole complexes was first observed by Chatfield and Armstrong [9]. This enhancement was not affected by anaerobic conditions, showing that the copper-imidazole complexes are substitutes for oxygen. Recently, the acti-vation of cytokinin oxidase by some phenolic compounds WebCytokinin measurements in ckx3 mutants confirmed that CKX3 activity negatively regulates root cytokinin levels. Particularly, tZ and DHZ type cytokinins in both inoculated and uninoculated roots were elevated in ckx3 mutants, suggesting that these are targets for degradation by the CKX3 cytokinin oxidase/dehydrogenase. The effect of CKX3 on the ... react fast uk ltd

Cytokinin dehydrogenase differentially regulates cytokinin and ...

WebSep 16, 2015 · Cytokinin dehydrogenase activity was measured using a modified end-point method described earlier . The reaction mixture (total volume of 0.6 ml in 1.5 ml tube) consisted of 100 mM McIlvaine buffer, pH 5.0 or 7.5, electron acceptor, substrate (dissolved in dimethyl sulfoxide that reached the final concentration of 2.5% in the reaction mixture ... WebNov 29, 2024 · Cytokinins have opposing actions in shoot and root growth—actions shown to involve cytokinin dehydrogenase (CKX), … react fallback componentWebJun 1, 2024 · The overexpression of CycD3 is sufficient to induce cytokinin-independent shoot formation in the calli 98, and the loss of CycD3 activity reduces the ability of exogenous cytokinins to induce ... how to start family history

"WebCytokinin dehydrogenase (CKX; EC.1.5.99.12) regulates the level of cytokinin (CK) in plants and is involved in CK regulatory activities. In different plants, a small gene family encodes CKX proteins with varied numbers of members. These genes are expanded in the genome mainly due to segmental duplication events. Despite their biological importance, … " - Cytokinin dehydrogenase activity

Cytokinin dehydrogenase activity

Cytokinin oxidase/dehydrogenase inhibitors stimulate 2iP to …

WebIntroductionZinc (Zn) as an essential micronutrient and cytokinin as phytohormone not only regulate plant growth but also play fundamental roles in plant tolerance against drought stress. Understating the function and the role of cytokinin in WebThe cytokinin dehydrogenases (CKX; EC 1.5.99.12) are a protein family that maintains the endogenous levels of cytokinins in plants by catalyzing their oxidative degradation. The CKX family in maize (Zea mays L.) has thirteen members, only two of which - ZmCKX1 and ZmCKX10 - have previously been characterized in detail.

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WebApr 26, 2024 · Cytokinin oxidase/dehydrogenase enzymes (CKX) catalyse the degradation of cytokinin by cleaving the active cytokinin form’s unsaturated … WebMar 30, 2024 · T6P has also emerged as an important cellular cue (Fichtner & Lunn, 2024), whereas pyruvate dehydrogenase kinase (PDK) has been implicated in metabolic quiescence in mammalian stem cells and may integrate with the tuning of quiescence via mitochondrial activity (Olson et al., 2016; Takubo et al., 2013). In the following …

WebCytokinin degraded by CKX1 is required for cell division in the female gametophyte by modulating the expression of cell cycle genes (PubMed:35995110). 5 publications … Webcytokinin, any of a number of plant hormones that influence growth and the stimulation of cell division. Cytokinins are synthesized in the roots and are usually derived from …

WebApr 9, 2024 · N 6-(2-Isopentenyl)adenine (2iP) and zeatin are natural N 6-substituted purine cytokinins that are used for in vitro culture protocols, mainly to promote axillary shoot proliferation and adventitious shoot induction.One of the mechanisms that regulates local cytokinin homeostasis in the in vitro plant is their degradation by cytokinin … WebMay 6, 2024 · Inhibitors of cytokinin oxidase/dehydrogenase (CKX) reduce the degradation of cytokinins in plants, and this effect can be exploited in agriculture and in plant tissue culture. In this study, we examine the structure–activity relationship of two series of CKX inhibitors based on diphenylurea.

WebJun 16, 2024 · a Cytokinin dehydrogenase activity in young panicles of WT and Osckx2-2 mutant. b Cytokinin content in the young panicles of WT and Osckx2-2 mutant. All data represented as Mean ± SD (n = 3). **A significant difference at the 0.01 level by the Student’s t test. Full size image.

WebCytokinin group of phytohormones regulate root elongation and branching during post‐embryonic development. Cytokinin degrading enzymes cytokinin … how to start fangs of the father questWebMar 4, 2014 · We have developed a N 6-dimethylallyladenine (cytokinin) dehydrogenase-based microbiosensor for real-time determination of the family of hormones known as cytokinins.Cytokinin dehydrogenase from Zea mays (ZmCKX1) was immobilised concurrently with electrodeposition of a silica gel film on the surface of a Pt … react fastclickWebThe size and activity of the shoot apical meristem is regulated by transcription factors and low molecular mass signals, including the plant hormone cytokinin. The cytokinin status of the meristem depends on different factors, including metabolic degradation of the hormone, which is catalyzed by cytokinin oxidase/dehydrogenase (CKX) enzymes. react famousWebMar 24, 2010 · Almost 80% of 52 regenerated lines of Golden Promise exhibited significantly decreased cytokinin oxidase/dehydrogenase (CKX) enzyme activity in bulked samples of their T 1 roots. There was a positive correlation between the enzyme activity and the plant productivity, expressed as the yield, the number of seeds per plant, and the 1000 grain … how to start fanslyWebSep 28, 2007 · The biological activity of cytokinin nucleotides is still unclear, since they usually show no activity in bioassays but can bind to certain cytokinin receptors … react faviconWebThe cytokinin zeatin is named after the genus of corn, Zea. Cytokinins ( CK) are a class of plant hormones that promote cell division, or cytokinesis, in plant roots and shoots. They … how to start fan in laptopWebJan 5, 2024 · Cytokinin regulates the activity of reproductive meristems, flower organ size, ovule formation, and thus seed yield in Arabidopsis thaliana. Bartrina I, et al. Plant Cell, … how to start far harbor dlc